Development of a sensor for L-Dopa based on Co(DMG)₂ClPy/multi-walled carbon nanotubes composite immobilized on basal plane pyrolytic graphite electrode

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• 作者：Fernando Roberto Figueirê ; do Leite^a  ; [Author Vitae] ; Camila Marchetti Maroneze^b  ; [Author Vitae] ; Adriano Bof de Oliveira^c  ; [Author Vitae] ; Wallans Torres Pio dos Santos^d  ; [Author Vitae] ; Flavio Santos Damos^a ; ^{flavio_damos@hotmail.com}  ; [Author Vitae] ; Rita de Cá ; ssia Silva Luz^a ; ^{rita_rcsluz@yahoo.com.br}  ; [Author Vitae]
• 关键词：L-Dopa oxidation ; Sensor ; Chloro(pyridine)bis(dimethylglyoximato)cobalt(III) ; Multi-walled carbon nanotube ; Basal plane pyrolytic graphite
• 刊名：Bioelectrochemistry
• 出版年：2012
• 出版时间：August, 2012
• 年：2012
• 卷：86
• 期：Complete
• 页码：22-29
• 全文大小：701 K

文摘

L-Dopa is the immediate precursor of the neurotransmitter dopamine, being the most widely prescribed drug in the treatment of Parkinson's disease. A sensitive and selective method is presented for the voltammetric determination of L-Dopa in pharmaceutical formulations using a basal plane pyrolytic graphite (BPPG) electrode modified with chloro(pyridine)bis(dimethylglyoximato)cobalt(III) (Co(DMG)₂ClPy) absorbed in a multi-walled carbon nanotube (MWCNT). Scanning Electron Microscopy and Fourier Transform Infrared Spectroscopy were used to characterize the materials. The electrocatalytical oxidation of L-Dopa using the Co(DMG)₂ClPy/MWCNT/BPPG electrode was investigated by cyclic voltammetry and square wave voltammetry. The parameters that influence the electrode response (the amount of Co(DMG)₂ClPy and of MWCNT, buffer solution, buffer concentration, buffer pH, frequency and potential pulse amplitude) were investigated. Voltammetric peak currents showed a linear response for L-Dopa concentration in the range of 3 to 100 ¦ÌM, with a sensitivity of 4.43 ¦ÌAcm^?#xA0;2/¦ÌM and a detection limit of 0.86 ¦ÌM. The related standard deviation for 10 determinations of 50 ¦ÌM L-Dopa was 1.6 % . The results obtained for L-Dopa determination in pharmaceutical formulations (tablets) were in agreement with the compared official method. The sensor was successfully applied for L-Dopa selective determination in pharmaceutical formulations.