- 作者：P.C. Kreuz ; C. Gentili ; B. Samans ; D. Martinelli ; J.P. Kr眉ger ; W. Mittelmeier ; M. Endres ; R. Cancedda ; C. Kaps
- 关键词：Cartilage regeneration ; Polyglycolic acid scaffold ; Tissue engineering ; Infant chondrocytes ; Hip cartilage repair
- 刊名：Osteoarthritis and Cartilage
- 出版年：December, 2013
- 年：2013
- 卷：21
- 期：12
- 页码：1997-2005
- 全文大小：2846 K
Summary
Objective
Studies about cartilage repair in the hip and infant chondrocytes are rare. The aim of our study was to evaluate the use of infant articular hip chondrocytes for tissue engineering of scaffold-assisted cartilage grafts.Method
Hip cartilage was obtained from five human donors (age 1-10 years). Expanded chondrocytes were cultured in polyglycolic acid (PGA)-fibrin scaffolds. De- and re-differentiation of chondrocytes were assessed by histological staining and gene expression analysis of typical chondrocytic marker genes. In聽vivo, cartilage matrix formation was assessed by histology after subcutaneous transplantation of chondrocyte-seeded PGA-fibrin scaffolds in immunocompromised mice.
Results
The donor tissue was heterogenous showing differentiated articular cartilage and non-differentiated tissue and considerable expression of type I and II collagens. Gene expression analysis showed repression of typical chondrocyte and/or mesenchymal marker genes during cell expansion, while markers were re-induced when expanded cells were cultured in PGA-fibrin scaffolds. Cartilage formation after subcutaneous transplantation of chondrocyte loaded PGA-fibrin scaffolds in nude mice was variable, with grafts showing resorption and host cell infiltration or formation of hyaline cartilage rich in type II collagen. Addition of human platelet rich plasma (PRP) to cartilage grafts resulted robustly in formation of hyaline-like cartilage that showed type II collagen and regions with type X collagen.
Conclusion
These results suggest that culture of expanded and/or de-differentiated infant hip cartilage cells in PGA-fibrin scaffolds initiates chondrocyte re-differentiation. The heterogenous donor tissue containing immature chondrocytes bears the risk of cartilage repair failure in聽vivo, which may be possibly overcome by the addition of PRP.