Preparation of redox-sensitive β-CD-based nanoparticles with controlled release of curcumin for improved therapeutic effect on liver cancer in vitro

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• 作者：Dae Hyeok Yang^a ; Hyun Joo Kim^a ; ^b ; Jae Kwang Kim^c ; Heung Jae Chun^a ; ^b ; ^{chunhj@catholic.ac.kr} ; Kyeongsoon Park^d
• 关键词：Beta-cyclodextrin nanoparticle (β-CD NP) system ; Curcumin ; Redox-sensitive ; Disulfide bond formation ; Liver cancer
• 刊名：Journal of Industrial and Engineering Chemistry
• 出版年：2017
• 出版时间：25 January 2017
• 年：2017
• 卷：45
• 期：Complete
• 页码：156-163
• 全文大小：2912 K
• 卷排序：45

文摘

A redox-sensitive beta-cyclodextrin nanoparticle (β-CD NP) system was prepared for the controlled release of curcumin (CUR), and its therapeutic efficacy on liver cancer was compared with that of CUR in vitro. 1-Dodecanethiol (DDT) was conjugated to per-6-thiol-β-CD by disulfide bond formation though an oxidation reaction (β-CD-ss-DDT). Rhodamine B (RhoB) was included into the β-CD-ss-DDT cavity by inclusion complex formation (β-CD-ss-DDT-ic-RhoB). CUR was encapsulated into β-CD-ss-DDT-ic-RhoB aggregates by self-assembly through dialysis method. The composition, morphology, size distribution and zeta potential of β-CD-ss-DDT-ic-RhoB NPs were characterized by proton nuclear magnetic resonance (1H NMR), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and dynamic light scattering (DLS), respectively. It was found that CUR was released from β-CD-ss-DDT-ic-RhoB/CUR NPs due to the dissociation of β-CD-ss-DDT through a reduction reaction. β-CD-ss-DDT-ic-RhoB/CUR NPs had a higher cellular uptake ratio and a greater anticancer effect on mouse hepatoma Hepa 1-6 cells than CUR, mainly attributed to the improved water-solubility of CUR after encapsulating it in the NPs. Our findings suggest that β-CD-ss-DDT-ic-RhoB NPs can be used as nanocarriers for delivering CUR into cancer cells, thereby serving as a clinical therapeutic agent for liver cancer treatment.