Development of a Real-Time PCR method for the identification of Atlantic mackerel (Scomber scombrus)
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- 作者：Amaya Velasco ; Ana S¨¢nchez ; Ic¨ªar Mart¨ªnez ; Francisco J. Santaclara ; Ricardo I. P¨¦rez-Mart¨ªn ; Carmen G. Sotelo
- 关键词：Atlantic mackerel ; Scomber ; Species identification ; Pelagic fish ; Real-Time PCR ; Cytochrome b
- 刊名：Food Chemistry
- 出版年：2013
- 出版时间：1 December, 2013
- 年：2013
- 卷：141
- 期：3
- 页码：2006-2010
- 全文大小：691 K
文摘
A Real Time-PCR method based on TaqMan technology for the identification of Scomber scombrus has been developed. A system of specific primers and a Minor Groove Binding (MGB) TaqMan probe based on sequences of the mitochondrial cytochrome b region was designed. The method was successfully tested in 81 specimens of S. scombrus and related species and validated in 26 different commercial samples. An average Threshold Cycle (Ct) value of 15.3 was obtained with S. scombrus DNA. With the other species tested fluorescence signal was not detected or Ct was significantly higher (P < 0.001). The efficiency of the assay was estimated to be 92.41 % , with 100 % specificity, and no cross reactivity was detected with any other species. These results reveal that the developed method is a rapid and efficient tool to unequivocally identify S. scombrus and may aid in the prevention of fraud or mislabelling in mackerel products.