Bioethanol from P. radiata is a promising alternative for fuel in Chile.

Organosolv catalyzed by an appropriate concentration of H₂SO₄ promotes structural and morphological changes in P. radiata, such as the formation of lignin droplets.

The formation of lignin droplets increases the digestibility of the pretreated material to achieve a high conversion of cellulose to glucose.

The proper dosage of last-generation enzyme Cellic Ctec^®3 to achieve high cellulose to glucose conversion is 0044 g of enzyme per g of dry pretreated material.

High ethanol concentrations and yields in dry wood basis were obtained through SSF and fed-batch SSF using the thermotolerant strain of Saccharomyces cereviseae IR2-9a at high substrate loadings.

With the feb-batch SSF strategy the viscosity of the formulations are kept low, and good mass transfer is maintained throughout the experiments.

From the results obtained by SSF, either in batch or fed-batch mode, it follows that high concentrations of ethanol are achieved thanks to the good enzymatic accessibility and digestibility of the pretreated material obtained.

Using the fed-batch SSF strategy increases the volumetric productivity (Q_P) of the reactions.

Cellulase Cellic Ctec^®3 enzyme complex maintains a suitable catalytic activity at 40 °C and is not affected by high concentrations of ethanol.

The highest ethanol concentration obtained was 85.01 g/L. This final concentration inhibits the fermentation by thermotolerant S. cerevisiae IR2-9a.