Surface-modified yeast cells: A novel eukaryotic carrier for oral application

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• 作者：Elisabeth E. Kenngott^a ; ¹ ; Ruth Kiefer^a ; Nicole Schneider-Daum^b ; Alf Hamann^c ; Marc Schneider^d ; Manfred J. Schmitt^a ; Frank Breinig^a ; ^{fb@microbiol.uni-sb.de" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Oral delivery ; Saccharomyces cerevisiae ; Cell surface-expression ; Invasin ; M cell
• 刊名：Journal of Controlled Release
• 出版年：2016
• 出版时间：28 February 2016
• 年：2016
• 卷：224
• 期：Complete
• 页码：1-7
• 全文大小：912 K

文摘

The effective targeting and subsequent binding of particulate carriers to M cells in Peyer's patches of the gut is a prerequisite for the development of oral delivery systems. We have established a novel carrier system based on cell surface expression of the β₁-integrin binding domain of invasins derived from Yersinia enterocolitica and Yersinia pseudotuberculosis on the yeast Saccharomyces cerevisiae. All invasin derivatives were shown to be effectively expressed on the cell surface and recombinant yeast cells showed improved binding to both human HEp-2 cells and M-like cells in vitro. Among the different derivatives tested, the integrin-binding domain of Y. enterocolitica invasin proved to be the most effective and was able to target Peyer's patches in vivo. In conclusion, cell surface-modified yeasts might provide a novel bioadhesive, eukaryotic carrier system for efficient and targeted delivery of either antigens or drugs via the oral route.