Dual Site Phosphorylation of Caspase-7 by PAK2 Blocks Apoptotic Activity by Two Distinct Mechanisms

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• 作者：Scott J. Eron¹ ; Kishore Raghupathi¹ ; Jeanne A. Hardy¹ ; ² ; ^{hardy@chem.umass.edu}
• 关键词：apoptosis ; cysteine protease ; kinase ; caspase ; prodomain ; phosphorylation-mediated inhibition ; phosphorylation ; kinase-caspase interaction ; steric block at active site ; phosphomimetic
• 刊名：Structure
• 出版年：2017
• 出版时间：3 January 2017
• 年：2017
• 卷：25
• 期：1
• 页码：27-39
• 全文大小：3915 K
• 卷排序：25

文摘

PAK2 phosphorylation of caspase-7 inhibits activity by two distinct mechanisms Phosphorylation of caspase-7 at S30 slows zymogen activation by upstream caspases S30 phosphorylation interferes with caspase-7:caspase-9 interaction Crystal structure of S239E phosphomimetic suggests substrate binding is obstructed