Expression analysis of ROS producing and scavenging enzyme-encoding genes in rubber tree infected by Pseudocercospora ulei
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- 作者：Daniela Martins Koopa ; Maryannick Riob ; Xavier Sabaub ; Saulo Emilio Almeida Cardosoc ; Chantal Cazevieilled ; Julie Leclercqb ; Dominique Garciab ; dominique.garcia@cirad.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Hevea ; Microcyclus ulei ; Oxidative burst ; Resistance ; SALB
- 刊名：Plant Physiology and Biochemistry
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：104
- 期：Complete
- 页码：188-199
- 全文大小：2150 K
文摘
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Three Hevea genotypes with contrasting resistances to SALB presented different behaviours in ROS regulation.
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Basal expression levels of HbRBOHA (low), HbCAT (low) and HbDHAR (high) indicated a balanced cellular redox state in MDF180.
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Pseudocercospora ulei infection induced a permanent up-regulation of the WRKY2 expression level in MDF180.
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In FX2784, up-regulation of HbRBOHD expression level and down-regulation of HbCAT indicated an oxidative burst at 48 hpi.
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In FX2784, signalisation (HbWRKY2), enzymatic ROS (HbGPX) and non-enzymatic ROS (HbGCL) elimination were also regulated at 48 hpi.
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In PB314, HbRBOHC, HbCuZnSOD cyto and HbCAT expression levels strengthened histo- and cytological observations at 216 hpi.