Speract, a sea urchin egg peptide that regulates sperm motility, also stimulates sperm mitochondrial metabolism
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- 作者：Juan Garcí ; a-Rincó ; n ; Alberto Darszon ; darszon@ibt.unam.mx" class="auth_mail" title="E-mail the corresponding author ; Carmen Beltrá ; n ; beltran@ibt.unam.mx" class="auth_mail" title="E-mail the corresponding author
- 关键词：ASW ; artificial sea water ; BCECF-AM ; 2&sbquo ; 7&sbquo ; -bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein ; acetoxymethylester ; [Ca2 ; +]i ; intracellular Ca2 ; + concentration ; CCCP ; carbonyl cyanide m-chlorophenylhydrazone ; CPT-I ; carnitine palmitoyl transferase-I ; 0CaASW ; is ASW lacking CaCl2 containing 30 μM EGTA yielding a final extracellular Ca2 ; + concentration ([Ca2 ; +]ext) of 110 nM ; ΔpHi ; intracellular pH change ; ΔΨ ; mitochondrial membrane potential ; Em ; membrane potential ; E
- 刊名：Biochimica et Biophysica Acta (BBA) - Bioenergetics
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：1857
- 期：4
- 页码：415-426
- 全文大小：1630 K
文摘
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Speract increases NADH levels and depolarizes the sea urchin sperm mitochondrion.
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The pHi increase triggered by speract finely coordinates the changes in ΔΨ and NADH.
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The speract-induced NADH rise and the ΔΨ decrease depend mainly on CPT-I activity.