Expression of holo-proteorhodopsin in Synechocystis sp. PCC 6803
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- 作者：Que Chena ; 1 ; Jeroen B. van der Steena ; 1 ; Henk L. Dekkerb ; Srividya Ganapathyc ; Willem J. de Gripc ; Klaas J. Hellingwerfa ; K.J.Hellingwerf@uva.nl" class="auth_mail" title="E-mail the corresponding author
- 关键词：DDM ; n-dodecyl-β-D-maltopyranoside ; pmf ; proton motive force ; PR(s) ; Proteorhodopsin(s) ; PR-His ; PR with a C-terminal poly-histidine tag ; PROPS ; proteorhodopsin optical proton sensor ; CM ; cytoplasmic membrane ; TM ; the thylakoid membrane ; SynACO ; apocarotenoid-15 ; 15&prime ; -oxygenase ; DCMU ; 3-(3 ; 4-dichlorophenyl)-1 ; 1-dimethylurea ; CCCP ; [(3-chlorophenyl) hydrazono] malononitrile
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：35
- 期：Complete
- 页码：83-94
- 全文大小：961 K
文摘
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Here we show that proteo-opsin can be expressed up to levels of 105 molecules per cell in membranes of Synechocystis sp. PCC6803.
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The heterologously expressed protein is equally distributed (based on amount of protein) over thylakoid- and cytoplasmic membranes.
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Synechocystis sp. PCC6803 is able to synthesize all-trans retinal and generate proteorhodopsin without requiring to add external retinal.
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Proteorhodopsin expressed in Synechocystis sp. PCC6803 can make a measurable contribution to light-energy conversion in this cyanobacterium.