A method for obtaining simian immunodeficiency virus RNA sequences from laser capture microdissected and immune captured CD68 + and CD163 + macrophages from frozen tissue sections of bone marrow and brain

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• 作者：Jaclyn Mallard^a ; Emily Papazian^a ; Caroline Soulas^a ; ^b ; David J Nolan^c ; ^d ; Marco Salemi^c ; ^d ; Kenneth C Williams^a ; ^{kenneth.williams.3@bc.edu}
• 关键词：Laser capture microdissection (LCM) ; Immunohistochemistry (IHC) ; Simian immunodeficiency virus (SIV) ; RNA
• 刊名：Journal of Immunological Methods
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：442
• 期：Complete
• 页码：59-63
• 全文大小：413 K
• 卷排序：442

文摘

A method for obtaining lentiviral RNA from LCM-captured laser capture micro dissected CD68 + and CD163 + macrophages A modified method using rapid immunohistochemistry in the presence of RNase-inhibitors