Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage

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• 作者：Yu-chao XU^a ; ^{1049205908@qq.com" class="auth_mail" title="E-mail the corresponding author} ; Xi-lin HOU^a ; Wei-wei XU^a ; Lu-lu SHEN^b ; Shan-wu LÜ ; ^a ; Shi-lin ZHANG^a ; Chun-mei HU^a ; ^{jjjhcm@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：non-heading Chinese cabbage ; stamen-petalody ; ethylene-responsive factor ; gene expression
• 刊名：Journal of Integrative Agriculture
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：15
• 期：3
• 页码：528-536
• 全文大小：1790 K

文摘

BrcERF-B3 gene, a member of ethylene-responsive factor family, was screened from a mutant plant in non-heading Chinese cabbage (Brassica rapa ssp. chinensis) by cDNA-AFLP technology. We got full length cDNA of two BrcERF-B3 genes by homology-based cloning from two materials and found that their nucleotide sequences were the same by sequencing. The BrcERF-B3 protein, belonging to the B3 subgroup of the ERF subfamily, shared a close relationship with B. rapa. RT-PCR result showed that BrcERF-B3 expressed only in mutant stamen rather than maintainer stamen. qRT-PCR results indicated that BrcERF-B3 expressed highly during reproductive growth development and in the early of mutant buds, suggesting BrcERF-B3 might be involved in the formation of abnormal flower in mutant. What's more, the expression of BrcERF-B3 was more significant to ABA, MeJA and cold stresses in mutant than in maintainer and was down-regulated in NaCl treatment in two lines, implying BrcERF-B3 might be different roles in biotic and abiotic stresses.