Simultaneous analysis of C1 and C4 oxidized oligosaccharides, the products of lytic polysaccharide monooxygenases acting on cellulose
详细信息 查看全文
- 作者：Bjø ; rge Westerenga ; bjorwe@nmbu.no" class="auth_mail" title="E-mail the corresponding author ; Magnus Ø ; . Arntzena ; Finn L. Aachmannb ; Anikó ; Vá ; rnaia ; Vincent G.H. Eijsinka ; Jane Wittrup Aggera
- 关键词：LC&ndash ; MS ; Carbohydrates ; Lytic polysaccharide monooxygenases ; Oxidized oligosaccharides ; Porous graphitized carbon chromatography ; High-performance anion exchange chromatography
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：6 May 2016
- 年：2016
- 卷：1445
- 期：Complete
- 页码：46-54
- 全文大小：1177 K
文摘
- •
PGC for quantitative and qualitative analysis of C1 and C4 oxidized cello-oligomers.
- •
C4-oxidized LPMO products undergo on-column decomposition during HPAEC analysis.
- •
Purification of C4-oxidized cello-oligomer standards.
- •
Determination of the pKa for cellobionic acid.
- •
Cello-oligomers are detected in the low ng range with charged aerosol detection.