Droplet digital polymerase chain reaction detection of HER2 amplification in formalin fixed paraffin embedded breast and gastric carcinoma samples

详细信息    查看全文

• 作者：Yazhen Zhu^a ; ¹ ; Dan Lu^b ; ¹ ; Maruja E. Lira^c ; ¹ ; Qing Xu^b ; Yunzhi Du^b ; Jianghong Xiong^b ; Mao Mao^b ; ^{mao_m@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Hyun Cheol Chung^d ; ^{UNCHUNG8@yuhs.ac" class="auth_mail" title="E-mail the corresponding author} ; Guangjuan Zheng^a ; ^{zhengguangjuan@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：HER2 amplification ; Droplet digital PCR ; Breast cancer ; Gastric cancer
• 刊名：Experimental and Molecular Pathology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：100
• 期：2
• 页码：287-293
• 全文大小：1112 K

文摘

Human epidermal growth factor receptor 2 (HER2) is a key driver of tumorigenesis, and over-expression as a result of HER2 gene amplification has been observed in a number of solid tumors. Recently HER2 has become an important biomarker for the monoclonal antibody treatment of HER2-positive metastatic breast and advanced gastric cancer. The HER2 targeting antibody trastuzumab treatment requires accurate measurement of HER2 levels for proper diagnosis. Droplet digital PCR (ddPCR) with highly direct, precise and absolute nucleic acid quantification could be used to detect HER2 amplification levels.

Objectives

Our objective was to evaluate a robust, accurate and less subjective application of ddPCR for HER2 amplification levels and test the assay performance in clinical formalin-fixed paraffin-embedded (FFPE) breast and gastric carcinoma samples.

Methods

Genomic DNA from HER2 amplified cell line SK-BR-3 was used to set up the ddPCR assays. The copy number of HER2 was compared to the chromosome 17 centromere reference gene (CEP17), expressed as HER2:CEP17 ratio. Genomic DNAs of FFPE specimens from 145 Asian patients with breast and gastric carcinomas were assayed using both standard methods, immunohistochemistry (IHC) and/or fluorescence in situ hybridization (FISH), and ddPCR.

Results

Based on 145 clinical breast and gastric carcinoma cases, our study demonstrated a high concordance of ddPCR results to FISH and IHC. In breast cancer specimens, the ddPCR results had high concordance with FISH and IHC defined HER2 status with a sensitivity of 90.9% (30/33) and a specificity of 100% (77/77). In gastric cancer specimens that were concordant in both FISH and IHC, our assay was 95.5% concordant with FISH and IHC (21/22).

Conclusions

ddPCR has the advantage of automation and also allows levels of HER2 amplification to be easily evaluated in large numbers of samples, and presents a potential option to define HER2 status.