The modification of PANI/GQD on SPCE was very simple and automated.
The determination of Cr(VI) using the modified SPCE has been entirely investigated.
The PANI/GQD-modified SPCE can be used several times without a renewal process.
The developed method provided high sensitivity with low sample consumption (0.5 mL).
The highest sample-throughput was found to be greater than 90 samples per hour.