Determination of acyl-CoA esters and acyl-CoA synthetase activity in mouse brain areas by liquid chromatography-electrospray ionization-tandem mass spectrometry

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• 作者：Fumiyo Kasuya ; ^{kasuya@pharm.kobegakuin.ac.jp} ; Teiichi Masuyama ; Taku Yamashita ; Kazuo Nakamoto ; Shougo Tokuyama ; Hiromi Kawakami
• 关键词：Acyl-CoAs ; Acyl-CoA synthetase ; Brain ; PUFA ; LC&ndash ; ESI-MS/MS
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2013
• 出版时间：15 June, 2013
• 年：2013
• 卷：929
• 期：Complete
• 页码：45-50
• 全文大小：863 K

文摘

The acyl-CoA levels and the acyl-CoA synthetase activities in 7 areas of mouse brain were determined by liquid chromatography-electrospray ionization-tandem mass spectrometry. Twenty-one acyl-CoA esters of C_2:0, C_4:0, C_6:0, C_8:0, C_10:0, C_12:0, C_14:1, C_14:0, C_16:0, C_16:1, C_18:0, C_18:1, C_18:2, C_18:3, C_20:0, C_20:4, C_20:5, C_22:0, C_22:5, C_22:6 and C_24:0 were detected in the olfactory bulb, cerebral cortex, hippocampus, cerebellum, hypothalamus, midbrain and medulla oblongata. The brain areas contained primarily the acyl-CoAs of the C_16:0, C_18:0, C_18:1, C_20:4 and C_22:6 species. The relative abundances of the acyl-CoAs of C_16:0, C_18:0 and C_18:1 were considerably higher than those of C_20:4 and C_22:6. The levels of medium-chain acyl-CoAs were only 1.2 % that of the long-chain acyl-CoAs. The differences in the acyl-CoA synthetase activities in each area of mouse brain were less dramatic. The order of the acyl-CoA synthetase activities for fatty acids of different chain lengths was palmitic acid > arachidonic acid > docosahexaenoic acid > octanoic acid. The analytical method proved to be very useful for the analysis of the acyl-CoA profile of tissues. Our results have important implications for understanding the regulation of acyl-CoA synthetase activity and long-chain fatty acid turnover in the phospholipids in the brain.