KPNA3-knockdown eliminates the second heat shock protein peak associated with the heat shock response of male silkworm pupae (Bombyx mori) by reducing heat shock factor transport into the nucleus
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- 作者：Jun Lia ; b ; Guoqing Weia ; Lei Wanga ; Cen Qiana ; Kedong Lia ; Congfen Zhanga ; Lishang Daia ; Yu Suna ; Dongran Liua ; Baojian Zhua ; zhubaojian@ahau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Chaoliang Liua ; cyschx@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：cNLS ; classic nuclear location sequence ; HSF ; heat shock transcription factor ; HSE ; heat shock element ; HSP ; heat shock protein ; KPNA3 ; Karyopherin alpha 3 ; RNAi ; RNA interference ; HSFi ; HSF specific RNA interference ; KPNA3i ; KPNA3 specific RNA interference ; NC ; negative control
- 刊名：Gene
- 出版年：2016
- 出版时间：10 January 2016
- 年：2016
- 卷：575
- 期：2
- 页码：452-457
- 全文大小：842 K
文摘
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We investigated the mechanism of the heat shock response in male silkworm pupae.
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We discovered two HSP mRNA peaks associated with the heat shock response.
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The second peak was eliminated by knockdown of KPNA3 or HSF.
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KPNA3 was shown to interact with HSF in vitro.