Methods for the physical characterization and quantification of extracellular vesicles in biological samples

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• 作者：Dé ; borah L.M. Rupert^a ; Virginia Claudio^a ; ^b ; Cecilia Lä ; sser^c ; Marta Bally^a ; ^d ; ^{bally@chalmers.se}
• 关键词：AFM ; atomic force microscopy ; BCA ; bicinchoninic acid ; Cryo-TEM ; cryo-transmission electron microscopy ; DLS ; dynamic light scattering ; dn/dC ; refractive index increment with concentration ; ELISA ; enzyme-linked immunosorbent assays ; EM ; electron microscopy ; EV ; extracellular vesicle ; FF-TEM ; freeze-fractioning transmission electron microscopy ; FSC ; forward scatter ; LSPR ; localized surface plasmon resonance ; MALS ; multi-angle light scattering ; mRNA ; messenger RNA ; NTA ; nanoparticle tracking analys
• 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：1861
• 期：1
• 页码：3164-3179
• 全文大小：1393 K
• 卷排序：1861

文摘

Characterizing exosomes is challenging due to their physicochemical heterogeneity. We present state-of-the-art methods to characterize and quantify vesicle samples. Physical properties discussed include size, density, optical properties and charge. Vesicle concentration is expressed in biomolecular content, particle number or mass. Profiling of extracellular vesicles requires complementary characterization tools.