Cytochemical Characterization of Members of the GenusFusobacteriumby Cellular Carbohydrate Fingerprints and Selected Phenotypic Features: TAXONOMY/SYSTEMATICS
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文摘
Members of the genusFusobacteriumare associated with several human diseases especially with periodontitis and gingivitis. Unfortunately, most of the current identification methods are insufficient for the correct identification of all the species in the genus, which makes it difficult to determine the etiological role of these bacteria in periodontal diseases. Chemotaxonomical markers for precise species description are still missing. The aim of our study was to investigate the applicability of cellular carbohydrate analysis for chemotaxonomic and diagnostic purposes. Forty strains ofFusobacteriumand one strain ofEubacterium plauti(formerlyFusobacterium plauti), as well as five strains ofBacteroides fragilisisolated from humans and animals were characterized by cytochemical features. The cellular carbohydrate profiles established by gas-liquid chromatography and mass spectrometry of the peracetylated aldononitriles and peracetylated methyloximes exhibited characteristic patterns suitable for the differentiation of four major groups within the genus. For further differentiation on the species level we used selected reactions of commercial test systems (API-ZYM and Rapid ID-32A) beside the carbohydrate analysis. Thus, we propose the use of cellular carbohydrate profiles in combination with selected phenotypic features for the identification of fusobacteria.