An on-line high-performance liquid chromatography−diode-array detector−multi-stage mass spectrometry−deoxyribonucleic acid−4′,6-diamidino-2-phenylindole−fluorescence detector system for screening the DNA-binding active compounds in Fufang Banbianlian Injection
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- 作者：Sensen Lia ; Haixiu Jianga ; Zongtao Linb ; Shanshan Dengc ; Yanqing Guana ; Hong Wanga ; ; Shizhong Chena ; chenbjmu@163.com" class="auth_mail" title="E-mail the corresponding author ; chenshizhong66@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：DAPI ; DNA binding activity ; Fufang Banbianlian Injection ; Fluorescence detector ; HPLC&minus ; DAD&minus ; MSn&minus ; DNA&minus ; DAPI&minus ; FLD ; On-line method
- 刊名：Journal of Chromatography A
- 出版年：2015
- 出版时间：11 December 2015
- 年：2015
- 卷：1424
- 期：Complete
- 页码：37-50
- 全文大小：1921 K
文摘
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An HPLC−DAD−MSn−DNA−DAPI−FLD system was established.
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21 of 58 identified compounds were active components in Fufang Banbianlian Injection.
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DAPI was used to screen ATT-specific DNA-binding compounds for the first time.
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The structure–activity relationships of different types of compounds were studied.
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MS fragmentation pathways were summarized in detail.