Comprehensive identification of 125 multifarious constituents in Shuang-huang-lian powder injection by HPLC-DAD-ESI-IT-TOF-MS

详细信息    查看全文

• 作者：Hongyang Sun^a ; ^{shy19880610@163.com" class="auth_mail" title="E-mail the corresponding author} ; Meixian Liu^a ; Zongtao Lin^b ; Haixiu Jiang^a ; Yanyan Niu^a ; Hong Wang^a ; ; Shizhong Chen^a ; ^{chenbjmu@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Flavonoids ; HPLC-DAD-ESI-IT-TOF-MS ; Iridoid glycosides ; Phenolic acids ; Phenylethanoid glycosides ; Shuang&ndash ; huang&ndash ; lian powder injection
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2015
• 出版时间：10 November 2015
• 年：2015
• 卷：115
• 期：Complete
• 页码：86-106
• 全文大小：1063 K

文摘

A high-performance liquid chromatography-diode array detector-electrospray ionization-ion trap-time of flight-mass spectrometry (HPLC-DAD-ESI-IT-TOF-MS) method was established for excellent separation and structural identification of constituents in Shuang–huang–lian powder injection (SHLPI). The typical ultraviolet absorptions, accurate empirical molecular formula and reasonable fragmentation mechanisms of these ingredients were used for their structural elucidation. In consequence, 125 constituents (33 phenolic acids, 29 flavonoids, 32 phenylethanoid glycosides, 15 iridoid glycosides, 8 lignans, 3 amino acids and 2 purines nucleosides, 2 quinoid glycosides and 1 alkylbenzene glycoside) were either unequivocally identified or tentatively characterized by comparing authentic standards or published data. The result showed that this study could provide valuable information for the quality control and further investigation of SHLPI formula.