Simultaneous quantification of chlorogenic acid and taurocholic acid in human plasma by LC-MS/MS and its application to a pharmacokinetic study after oral administration of Shuanghua Baihe tablets

详细信息    查看全文

• 作者：Pan GU^a ; ^b ; ^Δ ; Rui-Juan LIU^a ; ^b ; ^Δ ; Min-Lu CHENG^a ; ^b ; Yao WU^a ; ^b ; Lu ZHENG^c ; Yu-Jie LIU^c ; Peng-Cheng MA^d ; ^{mpc815@163.com" class="auth_mail" title="E-mail the corresponding author} ; Li DING^a ; ^b ; ^{dinglidl@hotmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Chlorogenic acid ; Taurocholic acid ; Shuanghua Baihe tablets ; Pharmacokinetics ; LC-MS/MS
• 刊名：Chinese Journal of Natural Medicines
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：14
• 期：4
• 页码：313-320
• 全文大小：858 K

文摘

An LC-MS/MS method was developed and validated for the simultaneous quantification of chlorogenic acid (CGA) and taurocholic acid (TCA) in human plasma using hydrochlorothiazide as the internal standard. The chromatographic separation was achieved on a Hedera ODS-2 column with a gradient elution using 10 mmol·L⁻¹ of ammonium acetate buffer solution containing 0.5% of formic acid – acetonitrile as mobile phase at a flow rate of 300 μL·min⁻¹. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring in negative ESI mode. The method was fully validated over the concentration ranges of 0.1–10 ng·mL⁻¹ for CGA and 2–150 ng·mL⁻¹ for TCA. It was successfully applied to a pharmacokinetic study of CGA and TCA in healthy Chinese volunteers after oral administration of Shuanghua Baihe tablets (SBTs). In the single-dose study, the maximum plasma concentration (C_max), time to reach C_max (T_max) and elimination half-life (t_1/2) of CGA were (0.763 8 ± 0.542 0) ng·mL⁻¹, (1.0 ± 0.5) h, and (1.3 ± 0.6) h, respectively. In the multiple-dose study, the C_max, T_max and t_1/2 of CGA were (0.663 7 ± 0.583 3) ng·mL⁻¹, (1.1 ± 0.5) h, and (1.4 ± 0.7) h, respectively. For TCA, no significant characteristic increasing plasma TCA concentration-time curve was found in the volunteers after oral administration of SBTs, indicating its complicated process in vivo as an endogenous ingredient.