MiRNA-183-5p promotes cell proliferation and inhibits apoptosis in human breast cancer by targeting the PDCD4

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• 作者：Yan Cheng^a ; ^{chengyan19820509@163.com" class="auth_mail" title="E-mail the corresponding author} ; Guixian Xiang^a ; Yanbo Meng^a ; Runzhi Dong^b
• 关键词：miR-183-5p ; PDCD4 ; Cell proliferation ; Cell cycle ; Apoptosis ; Breast cancer
• 刊名：Reproductive Biology
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：16
• 期：3
• 页码：225-233
• 全文大小：1601 K

文摘

MicroRNAs are often aberrantly expressed in breast cancer and postulated to play a causal role in the onset and maintenance of breast cancer by binding to its target mRNA. Here, we evaluated the effects of miRNA-183-5p on cell proliferation and apoptosis which attempted to elucidate the potential role of miR-183-5p/PDCD4 axis in human breast cancer. We found that the miR-183-5p expression level was extremely promoted in breast cancer in comparison with the adjacent normal tissues. Overexpression of miR-183-5p significantly enhanced the cell proliferation and inhibited cell apoptosis in MCF-7 and MDA-MB-231 cells. Moreover, PDCD4 was predicted as a putative target of miR-183-5p by bioinformatic approaches, and miR-183-5p negatively regulated the expression of PDCD4. Furthermore, knockdown of PDCD4 suppressed expression of p21 and p27, which was consistent with the result of the attachment of miR-183-5p. These data collectively demonstrate that miR-183-5p exerts oncomiRs effects in breast cancer, and may have broad impacts on the field of using antimiRs as anti-cancer drugs for breast cancer.