Two Supporting Factors Greatly Improve the Efficiency of Human iPSC Generation

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• 作者：Yang Zhao ; Xiaolei Yin ; Han Qin ; Fangfang Zhu ; Haisong Liu ; Weifeng Yang ; Qiang Zhang ; Chengang Xiang ; Pingping Hou ; Zhihua Song ; Yanxia Liu ; Jun Yong ; Pengbo Zhang ; Jun Cai ; Meng Liu ; Honggang Li ; Yanqin Li ; Xiuxia Qu ; Kai Cui ; Weiqi Zhang ; et al.
• 刊名：Cell Stem Cell
• 出版年：2008
• 出版时间：6 November 2008
• 年：2008
• 卷：3
• 期：5
• 页码：475-479
• 全文大小：496 K

文摘

Summary

Human fibroblasts can be induced into pluripotent stem cells (iPSCs), but the reprogramming efficiency is quite low. Here, we screened a panel of candidate factors in the presence of OCT4, SOX2, KLF4, and c-MYC in an effort to improve the reprogramming efficiency from human adult fibroblasts. We found that p53 siRNA and UTF1 enhanced the efficiency of iPSC generation up to 100-fold, even when the oncogene c-MYC was removed from the combinations.