High-level production of mature active-form Streptomyces mobaraensis transglutaminase via pro-transglutaminase processing using Streptomyces lividans as a host

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• 作者：Shuhei Noda ; Takaya Miyazaki ; Tsutomu Tanaka ; Ogino Chiaki ^{ochiaki@harbor.kobe-u.ac.jp} ; Akihiko Kondo ; ^{akondo@kobe-u.ac.jp}
• 关键词：Enzyme production ; Recombinant DNA ; Protease ; Filamentous bacteria ; Streptomyces mobaraensis transglutaminase ; Streptomyces lividans
• 刊名：Biochemical Engineering Journal
• 出版年：2013
• 出版时间：15 May, 2013
• 年：2013
• 卷：74
• 期：Complete
• 页码：76-80
• 全文大小：360 K

文摘

Streptomyces mobaraensis transglutaminase (MTG) is one of the most useful transglutaminases due to its rather broad substrate specificity and independence of Ca²⁺. To achieve efficient production of active-form MTG using Streptomyces lividans as a host, we created three vector constructs consisting of the signal peptide sequence (pld signal) derived from the phospholipase D gene of Streptomyces cinnamoneus, prepro-domain of S. cinnamoneus transglutaminase, and the sequence encoding mature MTG, and then generated three over-expressing S. lividans strains. We successfully demonstrated that S. lividans can be used as a host for the efficient production of mature, active-form MTG.