Peptidomic approach based on combined capillary isoelectric focusing and mass spectrometry for the characterization of the plasmin primary products from bovine and water buffalo β-casein
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- 作者：Andrea Somma ; Pasquale Ferranti ; Francesco Addeo ; Rosalba Mauriello ; Lina Chianese
- 关键词：Capillary electrophoresis ; LC/ESI-MS ; MALDI-TOF-MS ; Plasmin ; Casein ; Water buffalo
- 刊名：Journal of Chromatography A
- 出版年：2008
- 出版时间：30 May 2008
- 年：2008
- 卷：1192
- 期：2
- 页码：294-300
- 全文大小：817 K
文摘
The main peptides produced by hydrolysis of water buffalo β-casein with plasmin were characterized by capillary electrophoresis and mass spectrometry and compared with their bovine homologous. A novel breakdown product arising from the hydrolysis of water buffalo β-casein, originated by the presence of a plasmin-sensitive Lys bond at position 68 was identified, which was not present in bovine β-casein. On the basis of this evidence, an improved procedure for the detection and the differentiation of the products of plasmin hydrolysis of bovine and water buffalo β-casein by capillary isoelectric focusing was set-up. In the experimental conditions, the γ-casein from the two species was efficiently separated. Comparison of the capillary electropherograms with those obtained by ultra-thin-layer isoelectric focusing, the reference method for routine analysis of plasmin digests of casein, suggests that capillary electrophoresis isoelectric focusing may constitute a successful alternative to the traditional slab gel electrophoresis analysis of plasmin digests of casein either for basic structural studies or for applications in the quality assessment of dairy products.