LecT-Hepa: A triplex lectin–antibody sandwich immunoassay for estimating the progression dynamics of liver fibrosis assisted by a bedside clinical chemistry analyzer and an automated pretreatment machine

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• 作者：Atsushi Kuno^a ; Yuzuru Ikehara^a ; Yasuhito Tanaka^b ; Kozue Saito^a ; Kiyoaki Ito^c ; Chikayuki Tsuruno^d ; Shinya Nagai^d ; Youichi Takahama^d ; Masashi Mizokami^c ; Jun Hirabayashi^a ; Hisashi Narimatsu^a ; ^{h.narimatsu@aist.go.jp"" rel=""nofollow}
• 关键词：Alpha1-acid glycoprotein ; Biomarker ; Glycoprotein ; Hepatitis C virus ; Liver fibrosis ; Sandwich immunoassay
• 刊名：Clinica Chimica Acta
• 出版年：2011
• 出版时间：18 September 2011
• 年：2011
• 卷：412
• 期：19-20
• 页码：1767-1772
• 全文大小：325 K

文摘

Background

A quantitative analysis of glyco-alteration in serum glycoproteins provides glyco-parameters for estimating the progression of liver fibrosis. In the analysis of glycans, a manual pretreatment process for clinical specimens leads to a complicated manipulation and loss-of-clinical implementation of the assay.

Method

We evaluated an automated triplex lectin–antibody sandwich immunoassay assisted by an automated protein purification system (ED-01) and a bedside clinical chemistry analyzer (HISCL) for the acquisition of two glyco-parameters (AOL/DSA and MAL/DSA) derived from a fibrosis-related glyco-alteration of serum alpha1-acid glycoprotein (AGP).

Results

We adjusted the auto-machines with their accuracy set to CV <&#xa0;5.0 % (ED-01) and <&#xa0;1.0 % (HISCL). AGP samples were enriched from 275 serum specimens. Two glyco-parameters obtained by HISCL showed a linear correlation with that from a reported assay (R&#xa0;>&#xa0;0.90). The formula for monitoring fibrosis (LecT-Hepa) was given by a combination of the glyco-parameters. This correlated with the fibrosis stage from biopsy (R&#xa0;=&#xa0;0.68) and diagnosed severe fibrosis and cirrhosis. It was superior to that of FIB-4 index.

Conclusions

We automated a multilectin-assisted immunoassay with an order of magnitude reduction of operation time without any loss-of-accuracy. LecT-Hepa is a reliable method to assess fibrosis-dynamics from moderate fibrosis to cirrhosis.