Radiolabelled 5′-iodo-2′-deoxyuridine: a promising alternative to [¹⁸F]-2-fluoro-2-deoxy-d-glucose for PET studies of early response to anticancer treatment

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• 作者：Carnochan ; P. ; Brooks ; R.
• 关键词：Iododeoxyuridine ; Radiotracer ; PET ; Cell proliferation ; Pharmacodynamics
• 刊名：Nuclear Medicine and Biology
• 出版年：1999
• 出版时间：August, 1999
• 年：1999
• 卷：26
• 期：6
• 页码：667-672
• 全文大小：314 K

文摘

We investigated the potential of radiolabelled 5-iodo-2′-deoxyuridine (IUdR) as a pharmacodynamic probe for use with positron emission tomography (PET) in studies of early proliferative response to anticancer treatment. Using the hormone-responsive rat mammary carcinoma OES.HR1, we used a multiple radiotracer method to examine treatment-induced changes in 24 h tumour retention of [¹³¹I]IUdR, uptake of [³H]2-deoxy-d-glucose ([³H]DG) together with [^99mTc]hexylmethylpropylene amineoxine ([^99mTc]HMPAO) uptake as a measure of blood flow. Radiotracer data were compared with macroscopic changes in tumour growth, and cell proliferation as determined by DNA histogram flow cytometry. From 4 days after tumour growth arrest induced by oestrogen ablation, a sustained fall in tumour cell proliferation was demonstrated, which was associated with reduced tumour uptake of each tracer. Whereas reduced levels of tumour [³H]DG could be accounted for by changes in blood flow, this was not the case for [¹³¹I]IUdR, which was found to be closely related to percentage S-phase cells within tumour ( r