PAF and its metabolic enzymes in healthy volunteers: Interrelations and correlations with basic characteristics

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• 作者：Paraskevi Detopoulou^a ; ¹ ; Tzortzis Nomikos^a ; Elizabeth Fragopoulou^a ; George Stamatakis^b ; Demosthenes B. Panagiotakos^a ; Smaragdi Antonopoulou^a ; ^{antonop@hua.gr}
• 关键词：PAF ; platelet-activating factor ; lyso-PAF-AT ; acetyl-CoA ; lyso-PAF acetyltransferase ; PAF-CPT ; DTT-insensitive ; CDP-choline ; 1-alkyl-2-acetyl-sn-glycerol cholinephosphotransferase ; PAF-AH ; PAF-acetylhydrolase ; Lp-PLA2 ; lipoprotein-associated phospholipase-
• 刊名：Prostaglandins and Other Lipid Mediators
• 出版年：2012
• 出版时间：January 2012
• 年：2012
• 卷：97
• 期：1-2
• 页码：43-49
• 全文大小：260 K

文摘

PAF (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent inflammatory mediator, is synthesized via the remodeling and the de novo route, key enzymes of which are acetyl-CoA:lyso-PAF acetyltransferase (lyso-PAF-AT) and DTT-insensitive CDP-choline:1-alkyl-2-acetyl-sn-glycerol cholinephosphotransferase (PAF-CPT), respectively. PAF-acetylhydrolase (PAF-AH) and its extracellular isoform lipoprotein-associated phospholipase-A₂ (Lp-PLA₂) catabolize PAF. This study evaluated PAF levels together with leukocyte PAF-CPT, lyso-PAF-AT, PAF-AH and Lp-PLA₂ activities in 106 healthy volunteers. Men had lower PAF levels and higher activity of both catabolic enzymes and lyso-PAF-AT than women (P-values <0.05). Age was inversely correlated with PAF levels in men (r = ?.279, P = 0.06) and lyso-PAF-AT in women (r = ?.280, P = 0.05). In contrast, Lp-PLA₂ was positively correlated with age (r = 0.201, P = 0.04). Moreover, PAF-CPT was positively correlated with glucose (r = 0.430, P = 0.002) in women. In addition, Principal Component Analysis revealed three PAF metabolic patterns: (i) increased activities of PAF-CPT and PAF-AH, (ii) increased activities of PAF-CPT and lyso-PAF-AT and (iii) increased activity of Lp-PLA₂. The present study underlines the complexity of PAF's metabolism determinants.