- 作者：Miriam Mints ; Bo Blomgren ; Christian Falconer ; Aino Fianu-Jonasson ; Jan Palmblad
- 关键词：Angiogenesis ; endometrium ; menorrhagia ; receptors ; VEGF
- 刊名：Fertility and Sterility
- 出版年：2005
- 出版时间：September 2005
- 年：2005
- 卷：84
- 期：3
- 页码：692-700
- 全文大小：430 K
Objective
To analyze the expression of vascular endothelial growth factor A (VEGF-A) and receptors (VEGFR-1 and VEGFR-2) in endometrial blood vessels, as well as microvascular density (MVD), in endometrial biopsy samples from idiopathic menorrhagia patients.Design
Prospective clinical study.
Setting
University hospital, unit of gynecology.
Patient(s)
Twenty-four patients with idiopathic menorrhagia and 18 healthy fertile women.
Intervention(s)
Blood sampling for hormone measurement, hysteroscopy, and endometrial biopsy sampling. Endometrial biopsy samples were used for immunohistochemistry assessments and image analysis of stained endothelial structures for VEGF-A, VEGFR-1, VEGFR-2, and CD34.
Main Outcome Measure(s)
Appearance of the endometrial vascular immunoreactivity for VEGF-A, VEGFR-1, and VEGFR-2, MVD and computer-assisted stereological analysis of immunoassayed blood vessels.
Result(s)
Although the MVD did not differ between patients and controls, we observed that vascular expression of VEGF-A, VEGFR-1, and VEGFR-2 in capillaries was 1.8-fold, 1.8-fold, and 2.0-fold higher, respectively, in the menorrhagia group when assessed as the number of stained capillaries per unit area. There were also a twofold higher number of arterioles, which were VEGFR-2 positive in the menorrhagia group.
Conclusion(s)
Up-regulation of VEGF-A and receptors VEGFR-1 and VEGFR-2 in capillaries in menorrhagia could be involved in abnormal endometrial vascular structure and permeability.