Evaluation of non-conventional polar modifiers on immobilized chiral stationary phases for improved resolution of enantiomers by supercritical fluid chromatography
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- 作者：Jimmy O. DaSilva ; Br ; yce Coes ; Lisa Frey ; Ingrid Mergelsberg ; Ray McClain ; Lisa Nogle ; Christopher J. Welch
- 关键词：SFC ; Chiral ; Analytical ; Preparative ; Chromatography ; Columns
- 刊名：Journal of Chromatography A
- 出版年：7 February, 2014
- 年：2014
- 卷：1328
- 期：Complete
- 页码：98-103
- 全文大小：1216 K
文摘
An evaluation of the use of non-conventional polar modifiers for the supercritical fluid chromatographic separation of enantiomers on immobilized chiral stationary phases is presented. The resolution of a group of nine commercially available racemates is studied on the Chiralpak IA, IB, IC, ID, IE, and IF chiral stationary phases using CO2-based eluents containing non-conventional polar modifiers such as dichloromethane, chloroform, tetrahydrofuran, 2-methyl tetrahydrofuran, methyl tert-butyl ether, cyclopentyl methyl ether, acetone, ethyl acetate, toluene, 2,2,2-trifluoroethanol, and N,N-dimethylformamide. Screening experiments and method development for the commercial racemates on the immobilized columns with the non-conventional solvents demonstrated an ability to adjust the retention and improve resolution. From these results we were able to assign a general eluotropic relationship between the non-conventional solvents and methanol. A general ability to selectively adjust chromatographic retention while improving analyte solubility can lead to improved preparative chromatographic performance.