Detection of dehalogenation impurities in organohalogenated pharmaceuticals by UHPLC-DAD-HRESIMS

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• 作者：Erik L. Regalado ; ^{erik.regalado@merck.com" class="auth_mail} ; ^{erikluis18@gmail.com" class="auth_mail} ; Renee K. Dermenjian ; Leo A. Joyce ; Christopher J. Welch ; ^{christopher_welch@merck.com" class="auth_mail}
• 关键词：Dehalogenation impurities ; Method development ; Perfluorophenyl ; UHPLC ; HRMS
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：15 April, 2014
• 年：2014
• 卷：92
• 期：Complete
• 页码：1-5
• 全文大小：1129 K

文摘

The presence of dehalogenated impurities is often observed in halogen-containing pharmaceuticals, and can present a difficult analytical challenge, as the chromatographic behavior of the halogenated drug and the hydrogen-containing analog can be quite similar. In this study we describe the chromatographic separation and unambiguous identification of dehalogenation impurities or associated isomers in organohalogenated pharmaceuticals using UHPLC with a pentafluorophenyl column coupled with diode-array and high resolution electrospray ionization mass spectrometry detection (UHPLC-DAD-HRESIMS).