Effect of pressure on the chromatographic separation of enantiomers under reversed-phase conditions
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- 作者：Alexey A. Makarov ; alexey.makarov@merck.com" class="auth_mail ; almak2@gmail.com" class="auth_mail ; Erik L. Regalado ; Christopher J. Welch ; Wes A. Schafer
- 关键词：Pressure ; Enantiomer ; Chiral ; Ultra high pressure LC ; UHPLC
- 刊名：Journal of Chromatography A
- 出版年：25 July 2014
- 年：2014
- 卷：1352
- 期：Complete
- 页码：87-92
- 全文大小：1107 K
文摘
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During separation on chiral stationary phases an increase in pressure led to an increase of retention factors for some analytes and a decrease for others.
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During separations on achiral C-18 an increase in pressure always led only to an increase of retention factor.
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Changes in pressure led to small changes in enantioselectivity.
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Changes in enantioselectivity may be due to pressure effects on the alpha-helix groove size in chiral stationary phases based on cellulose or amylose.