Ethylene glycol assisted preparation of Ti4+-modified polydopamine coated magnetic particles with rough surface for capture of phosphorylated proteins
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- 作者：Xiangdong Ma ; Chun Ding ; Xin Yao ; Li Jia ; jiali@scnu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Ti4+-rPDA@Fe3O4 particles ; Separation ; Immobilized metal ion affinity chromatography ; Phosphorylated proteins
- 刊名：Analytica Chimica Acta
- 出版年：2016
- 出版时间：27 July 2016
- 年：2016
- 卷：929
- 期：Complete
- 页码：23-30
- 全文大小：1847 K
文摘
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Ti4+-rPDA@Fe3O4 with rough surface was synthesized by introduction of EG.
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The particles demonstrated good selectivity for phosphoproteins based on IMAC.
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The adsorption capacity of Ti4+-rPDA@Fe3O4 for κ-Cas was 1105.6 mg g−1.
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The particles were successfully applied for isolation of phosphoproteins in milk.