Dialdehyde cellulose-based supports evaluated for α-chymotrypsin immobilization.
α-Chymotrypsin was covalently immobilized via Schiff-base/imine linkage formation.
Immobilized chymotrypsin has reusability, storage, pH and temperature stability.
It has enhanced activity even under the simulated physiological conditions.
A good correlation of experimental data was predicted by ANN model.