High-level expression and characterization of two serine protease inhibitors from Trichinella spiralis
详细信息 查看全文
- 作者：Zhaoxia Zhanga ; Yixian Maoa ; Da Lia ; Yvhan Zhanga ; Wei Lia ; Honglin Jiab ; Jun Zhengb ; Li Lia ; Yixin Lua ; luyixin@neau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Trichinella spiralis ; Serine protease inhibitor ; Trypsin ; Chymotrypsin ; Pepsin
- 刊名：Veterinary Parasitology
- 出版年：2016
- 出版时间：30 March 2016
- 年：2016
- 卷：219
- 期：Complete
- 页码：34-39
- 全文大小：1451 K
文摘
- •
We have expressed two serine protease inhibitors, belonging to the TIL and serpin superfamily.
- •
Tsp03044 showed inhibitory effects on trypsin, α-chymotrypsin, and pepsin.
- •
TspAd5 could effectively inhibit the activities of α-chymotrypsin and pepsin.
- •
Tsp03044 and TspAd5 showed activity between 28 and 48 °C.
- •
Tsp03044 and TspAd5 were much more highly expressed at the ML phase.