Quantitative determination of active Bowman-Birk isoinhibitors, IBB1 and IBBD2, in commercial soymilks

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• 作者：M. Carmen Arques^a ; M. Carmen Marí ; n-Manzano^a ; L. Clarissa Brito da Rocha^b ; Blanca Hernandez-Ledesma^c ; Isidra Recio^c ; Alfonso Clemente^a ; ^{alfonso.clemente@eez.csic.es" class="auth_mail}
• 关键词：BAPNA ; N-伪-benzoyl-DL-arginine-p-nitroanilide ; BBI ; Bowman-Birk inhibitors ; BBIC ; Bowman-Birk inhibitor concentrate ; BTEE ; N-benzoyl-L-tyrosine ethyl ester ; CIA ; chymotrypsin inhibitor activity ; CIU ; chymotrypsin inhibitor units ; CRC ; colorectal cancer ; DMH ; dimethylhydrazine ; GIT ; gastrointestinal tract ; IU ; inhibitor units ; Ki ; inhibition constant ; KTI ; Kunitz trypsin inhibitor ; SM ; soymilk ; TIA ; trypsin inhibitor activity ; TIU ; trypsin inhibitor units
• 刊名：Food Chemistry
• 出版年：15 July, 2014
• 年：2014
• 卷：155
• 期：Complete
• 页码：24-30
• 全文大小：588 K

文摘

Naturally-occurring serine protease inhibitors of the Bowman-Birk family exert their potential chemopreventive and/or therapeutic properties via protease inhibition. In this study, we have quantified the amounts of active BBI isoinhibitors, IBB1 and IBBD2, in six commercial soymilks. By using cation exchange chromatography, the BBI isoinhibitors were isolated and their specific trypsin inhibitory activity was used to estimate their amounts in soymilk samples. IBB1 and IBBD2 concentrations ranged from 0.44 to 5.20 and 0.27 to 4.60 mg/100 ml of soymilk, respectively; total BBI, considered as the sum of both isoinhibitors, ranged from 0.60 to 9.07 mg/100 ml of soymilk. These data show that physiologically relevant amounts of active BBI are present in commercial soymilk and may exert potential health-promoting effects.