- 作者：Giuseppina Li Pira1 ; giuseppina.lipira@opbg.net" class="auth_mail" title="E-mail the corresponding author ; David Malaspina1 ; Elia Girolami1 ; Simone Biagini1 ; Elisabetta Cicchetti1 ; Gianpiero Conflitti1 ; Manuel Broglia1 ; Stefano Ceccarelli1 ; Stefania Lazzaro1 ; Daria Pagliara1 ; Antonella Meschini1 ; Alice Bertaina1 ; Mauro Montanari1 ; Franco Locatelli1 ; 2
- 关键词：HLA-haploidentical transplantation ; Graft manipulation ; αβ T cell depletion
- 刊名：Biology of Blood and Marrow Transplantation
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：22
- 期：11
- 页码：2056-2064
- 全文大小：643 K
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Removal of αβ T cells and B cells from the graft is a safe and effective strategy to perform T cell–depleted allogeneic hematopoietic stem cell transplantation from an HLA-haploidentical family donor
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We describe our 3-year experience with the αβ T cell and B cell depletion procedure, which was performed using clinical-grade reagents and an automated closed-system device
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Depletion efficiency of αβ T cells and of B cells was robust and reproducible, resulting into a median value of 4.1 logs (range, 3.1 to 5.5) and of 3.4 logs (range, 2.0 to 4.7), respectively
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Recovery of CD34+ cells showed a mean value of 90% (SD, 11%)
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Recommendations are provided to optimize procedure performance. In particular, we show that staff proficiency may help reduce cell loss during the initial manipulation phases