Potential of four marine-derived fungi extracts as anti-proliferative and cell death-inducing agents in seven human cancer cell lines

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• 作者：Alice Abreu Ramos¹ ; ^# ; Maria Prata-Sena¹ ; ² ; ^# ; Bruno Castro-Carvalho¹ ; ² ; Tida Dethoup³ ; Suradet Buttachon¹ ; ² ; Anake Kijjoa¹ ; ² ; Eduardo Rocha¹ ; ² ; ^{erocha@icbas.up.pt" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Anti-proliferative activity ; Antineoplastic agents ; Cell death ; Cytotoxicity ; Human cancer cell lines ; Marine-derived fungi
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：8
• 期：10
• 页码：798-806
• 全文大小：1038 K

文摘

To evaluate the in vitro anticancer activity of crude ethyl acetate extracts of the culture of four marine-derived fungi Aspergillus similanensis KUFA 0013 (E1), Neosartorya paulistensis KUFC 7897 (E2), Neosartorya siamensis KUFA 0017 (E4) and Talaromyces trachyspermus KUFC 0021 (E3) on a panel of seven human cancer cell lines.

id="absSec_2">Methods

id="abspara0015">Effects on cell proliferation, induction of DNA damage and cell death were assessed by MTT and clonogenic assays, comet assay and nuclear condensation assay, respectively.

id="absSec_3">Results

id="abspara0020">The proliferation of HepG2, HCT116 and A375 cells decreased after incubation with the extracts E2 and E4. The anti-proliferative effect was confirmed by morphologic alterations and by clonogenic assay. Both extracts also induced cell death in HepG2 and HCT116 cells. Doxorubicin was used as a positive control and showed in vitro anticancer activity.

id="absSec_4">Conclusions

id="abspara0025">This study demonstrated, for the first time, that extracts of Neosartorya paulistensis and Neosartorya siamensis have selective anti-proliferative and cell death activities in HepG2, HCT16 and A375 cells. The bioactivity of these extracts suggests a potential for biotechnological applications and substantiates that both should be further considered for the elucidation of the molecular targets and signal transduction pathways involved.