Effects of cryopreservation on the epigenetic profile of cells

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• 作者：A. Chatterjee^a ; ¹ ; D. Saha^a ; ¹ ; H. Niemann^b ; O. Gryshkov^a ; B. Glasmacher^a ; ^{glasmacher@imp.uni-hannover.de} ; N. Hofmann^a
• 关键词：Cryopreservation ; DNA methylation ; Histone post-translational modifications ; Cellular effects
• 刊名：Cryobiology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：74
• 期：Complete
• 页码：1-7
• 全文大小：629 K
• 卷排序：74

文摘

Effective cryopreservation protocols are essential for long-term storage of cells and their subsequent clinical application. Freezing protocols are generally considered as safe; however, putative effects on epigenetic marks have not yet been studied in detail. While post-thaw cell survival rates have been used to evaluate the success of cryopreservation protocols, increasing evidence suggests that freezing may be associated with deviations from the physiological epigenetic marks with putative long-term effects on the cells and/or their derivatives. A better understanding of the underlying mechanisms would be beneficial for improving safety and effectiveness of freezing protocols. The purpose of this review is to provide current information regarding epigenetic alterations (DNA methylation and histone modification patterns) associated with cryopreservation.