Non-replicating adenovirus vectors expressing avian influenza virus hemagglutinin and nucleocapsid proteins induce chicken specific effector, memory and effector memory CD8⁺ T lymphocytes

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• 作者：Shailbala Singh ; Haroldo Toro ; De-Chu Tang ; Worthie E. Briles ; Linda M. Yates ; Renee T. Kopulos ; Ellen W. Collisson
• 关键词：Avian influenza virus ; Chicken T lymphocytes ; Adenovirus vectors ; Influenza hemagglutinin ; Influenza nucleocapsid ; Memory T lymphocytes ; Effector T lymphocytes
• 刊名：Virology
• 出版年：2010
• 出版时间：15 September 2010
• 年：2010
• 卷：405
• 期：1
• 页码：62-69
• 全文大小：543 K

文摘

Avian influenza virus (AIV) specific CD8⁺ T lymphocyte responses stimulated by intramuscular administration of an adenovirus (Ad) vector expressing either HA or NP were evaluated in chickens following ex vivo stimulation by non-professional antigen presenting cells. The CD8⁺ T lymphocyte responses were AIV specific, MHC-I restricted, and cross-reacted with heterologous H7N2 AIV strain. Specific effector responses, at 10 days post-inoculation (p.i.), were undetectable at 2 weeks p.i., and memory responses were detected from 3 to 8 weeks p.i. Effector memory responses, detected 1 week following a booster inoculation, were significantly greater than the primary responses and, within 7 days, declined to undetectable levels. Inoculation of an Ad-vector expressing human NP resulted in significantly greater MHC restricted, activation of CD8⁺ T cell responses specific for AIV. Decreases in all responses with time were most dramatic with maximum activation of T cells as observed following effector and effector memory responses.