Creation of targeted inversion mutations in plants using an RNA-guided endonuclease

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• 作者：Congsheng Zhang^a ; ^b ; Changlin Liu^a ; Jianfeng Weng^a ; Beijiu Cheng^b ; Fang Liu^a ; Xinhai Li^a ; Chuanxiao Xie^a ; ^{xiechuanxiao@caas.cn}
• 关键词：RNA-guided endonuclease ; CRISPR/Cas9 ; Targeted inversion ; Genetic improvement
• 刊名：The Crop Journal
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：5
• 期：1
• 页码：83-88
• 全文大小：1037 K
• 卷排序：5

文摘

Inversions are DNA rearrangements that are essential for plant gene evolution and adaptation to environmental changes. We demonstrate the creation of targeted inversions and previously reported targeted deletion mutations via delivery of a pair of RNA-guided endonucleases (RGENs) of CRISPR/Cas9. The efficiencies of the targeted inversions were 2.6% and 2.2% in the Arabidopsis FLOWERING TIME (AtFT) and TERMINAL FLOWER 1 (AtTFL1) loci, respectively. Thus, we successfully established an approach that can potentially be used to introduce targeted DNA inversions of interest for functional studies and crop improvement.