Optimization of non-linear gradient in hydrophobic interaction chromatography for the analytical characterization of antibody-drug conjugates

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• 作者：Balá ; zs Bobá ; ly ; Giuseppe Marco Randazzo ; Serge Rudaz ; Davy Guillarme ; Szabolcs Fekete ; ^{szabolcs.fekete@unige.ch}
• 关键词：Hydrophobic interaction chromatography ; Antibody-drug-conjugate ; Method development ; Retention modelling ; Non-linear gradient
• 刊名：Journal of Chromatography A
• 出版年：2017
• 出版时间：20 January 2017
• 年：2017
• 卷：1481
• 期：Complete
• 页码：82-91
• 全文大小：1433 K
• 卷排序：1481

文摘

A concave logarithmic gradient was optimized for ADC DAR separations in HIC. The LSS retention model was extended for logarithmic scouting gradients. The accuracy of retention time prediction was found to be excellent.