- 作者：T. Yamamoto ; T. Uchiyama ; R. Sakakibara ; J. Taniguchi ; S. Kuwabara
- 关键词：5-HIAA ; 5-hydroxyindoleacetic acid ; 6-OHDA ; 6-hydroxydopamine ; DA ; dopamine ; DOPAC ; 3&ndash ; 4-dihydroxyphenylacetic acid ; EDTA ; ethylenediaminetetraacetic acid ; FFT ; fast Fourier transform ; H2O2 ; hydrogen peroxide ; HPLC ; high-performance liquid chromatography ; HVA ; Homovanillic acid ; LFP ; local field potential ; PBS ; phosphate-buffered saline ; PBST ; PBS containing 0.05% Tween-20 ; PD ; Parkinson&rsquo ; s disease ; PSD ; power spectral density ; SNc ; Substantia nigra pars compacta ; STN-DBS ; subthalamic
- 刊名：Neuroscience
- 出版年：14 February, 2014
- 年：2014
- 卷：259
- 期：Complete
- 页码：43-52
- 全文大小：1794 K
Aims
Not all the mechanisms by which subthalamic nucleus deep brain stimulation (STN-DBS) alleviates parkinsonian symptoms have been clarified as yet. The levels of striatal monoamine and the subthalamic beta activity might contribute to its efficacy. However, their direct relationship is unclear. We aimed to examine the correlation between the striatal monoamine and the STN beta activity induced by STN-DBS.Experimental procedures
Experiments were performed under urethane anesthesia in normal (n = 4) and 6-hydroxydopamine hemi-lesioned Parkinson鈥檚 disease (PD) model rats (n = 5). STN-DBS was applied to the left STN, and local field potential (LFP) was recorded before and after STN-DBS. Striatal extracellular fluid was collected before, during, and after STN-DBS. Spectral analysis of STN-LFP was performed, and the levels of monoamine were measured.
Results
The levels of 3-4-dihydroxyphenylacetic acid (DOPAC) were significantly decreased after the cessation of stimulation in PD model rats. The levels of none of the monoamines were significantly affected in normal rats. The STN beta power was significantly elevated after the cessation of stimulation in normal rats but was significantly decreased in PD model rats.
The STN beta power and the levels of DOPAC and 5-HT was positively correlated in PD model rats, whereas the levels of dopamine and 5-HT showed positive correlation and the levels of DOPAC and Homovanillic acid (HVA) showed negative correlation in normal rats.
Conclusion
STN-DBS could decrease the levels of DOPAC and the STN beta power in a PD model rat. The STN beta power and the levels of striatal monoamine might be differentially correlated between normal and PD model rats.