- 作者：Israel Castillo-Ju谩rez ; Rodolfo Garc铆a-Contreras ; Norma Vel谩zquez-Guadarrama ; Marcos Soto-Hern谩ndez ; Mariano Mart铆nez-V谩zquez
- 关键词：Anacardic acid ; Quorum-sensing inhibitors ; Amphipterygium adstringens ; Anti-quorum ; Antimicrobial plant
- 刊名：Archives of Medical Research
- 出版年：October, 2013
- 年：2013
- 卷：44
- 期：7
- 页码：488-494
- 全文大小：710 K
Background and Aims
Quorum sensing (QS) is a process of bacterial cell-cell communication that controls a large number of systems affecting pathogenicity. Interrupting this communication system can provide nonvirulent pathogenic bacteria. The aim of this study was to evaluate the anti-quorum sensing (anti-QS) potential of an anacardic acids mixture isolated from Amphipterygium adstringens, a medicinal plant known as 鈥渃uachalalate鈥? to prevent the onset of bacterial infections as an alternate to antibiotics.Methods
Initially we investigated the anti-QS activity of A. adstringens hexane extract (HE) by the inhibition of violacein production in Chromobacterium violaceum. From the active HE, an anacardic acid mixture (AAM) was obtained. The anti-quorum sensing activity of AAM was investigated by the rhamnolipid and pyocyanin production constraint as well as decrease of elastase activity, all being quorum sensing-controlled virulence factors expressed in the pathogenic bacteria Pseudomonas aeruginosa.
Results
HE induced a 91.6% of inhibition of the violecin production at 55 渭g/mL concentration, whereas AAM showed 94% of inhibition at 166 渭g/mL. In both cases, inhibition of violacein production did not affect the viability of the bacterium. AAM inhibited pyocyanin (86% at 200 渭g/mL) and rhamnolipid (91% at 500 渭g/mL) production in a dose/response form and decrease the elastase (75% at 500 渭g/mL) activity in P. aeruginosa without affecting its development.
Conclusions
Because an anacardic acids mixture isolated from A. adstringens demonstrated anti-QS, it could be further exploited for novel molecules to treat the emerging infections of antibiotic-resistant bacterial pathogens.