Multi-center evaluation of a user-friendly lateral flow assay to determine IP-10 and CCL4 levels in blood of TB and non-TB cases in Africa
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- 作者：Paul L.A.M. Corstjensa ; P.Corstjens@LUMC.NL" class="auth_mail" title="E-mail the corresponding author ; Elisa M. Tjon Kon Fata ; Claudia J. de Dooda ; Jolien J. van der Ploeg-van Schipb ; Kees L.M.C. Frankenb ; Novel N. Chegouc ; Jayne S. Sutherlandd ; Rawleigh Howee ; Adane Mihrete ; Desta Kassaf ; Marieta van der Vyverg ; Jacob Sheehamag ; Felanji Simukondah ; Harriet Mayanja-Kizzai ; Tom H.M. Ottenhoffb ; Gerhard Walzlc ; Annemieke Gelukb ; On behalf of the AE-TBC consortium1
- 关键词：CCL4 ; chemokine C&ndash ; C motif ligand 4 ; C/E ; ESAT-6/CFP-10 ; IFN-γ ; interferon gamma ; IP-10 ; IFN-γ induced protein 10 ; LTBI ; latent TB infection ; LF ; lateral flow ; LLOD ; lower limit of detection ; NEC ; non-endemic controls ; ORD ; other respiratory diseases ; QC ; quality control ; SOP ; standard operating procedure ; TB ; tuberculosis ; UCP ; up-converting phosphor
- 刊名：Clinical Biochemistry
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：49
- 期：1-2
- 页码：22-31
- 全文大小：1311 K
文摘
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Evaluation of user-friendly, quantitative, lateral flow tests for chemokines, IP-10 and CCL4
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Determination of chemokine levels in the range of 100 to 100,000 pg/mL in whole blood samples
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Multi-country study in Africa comparing TB and cases with other respiratory disease
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Demonstration of robustness and applicability of the low-tech UCP-LF test platform in Africa
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Dry reagent test materials allow convenient worldwide shipping and storage at ambient temperature