Inhibition of tumor growth and metastasis by photoimmunotherapy targeting tumor-associated macrophage in a sorafenib-resistant tumor model

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• 作者：Chenran Zhang^a ; Liquan Gao^a ; Yuehong Cai^b ; Hao Liu^a ; Duo Gao^a ; Jianhao Lai^a ; Bing Jia^a ; Fan Wang^a ; ^c ; Zhaofei Liu^a ; ^{liuzf@bjmu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Tumor-associated macrophage (TAM) ; Tumor microenvironment ; Molecular imaging ; Photoimmunotherapy ; Tumor metastasis
• 刊名：Biomaterials
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：84
• 期：Complete
• 页码：1-12
• 全文大小：4759 K

文摘

Tumor-associated macrophages (TAMs) play essential roles in tumor invasion and metastasis, and contribute to drug resistance. Clinical evidence suggests that TAM levels are correlated with local tumor relapse, distant metastasis, and poor prognosis in patients. In this study, we synthesized a TAM-targeted probe (IRD-αCD206) by conjugating a monoclonal anti-CD206 antibody with a near-infrared phthalocyanine dye. We then investigated the potential application of the IRD-αCD206 probe to near-infrared fluorescence (NIRF) imaging and photoimmunotherapy (PIT) of tumors resistant to treatment with the kinase inhibitor sorafenib. Sorafenib treatment had no effect on tumor growth in a 4T1 mouse model of breast cancer, but induced M2 macrophage polarization in tumors. M2 macrophage recruitment by sorafenib-treated 4T1 tumors was noninvasively visualized by in vivo NIRF imaging of IRD-αCD206. Small-animal single-photon emission computed tomography (SPECT)/CT and intratumoral microdistribution analysis indicated TAM-specific localization of the IRD-αCD206 probe in 4T1 tumors after several rounds of sorafenib treatment. Upon light irradiation, IRD-αCD206 suppressed the growth of sorafenib-resistant tumors. In vivo CT imaging and ex vivo histological analysis confirmed the inhibition of lung metastasis in mice by IRD-αCD206 PIT. These results demonstrate the utility of the IRD-αCD206 probe for TAM-targeted diagnostic imaging and treatment of tumors that are resistant to conventional therapeutics.