Chemical profiling of Qixue Shuangbu Tincture by ultra-performance liquid chromatography with electrospray ionization quadrupole-time-of-flight high-definition mass spectrometry (UPLC-QTOF/MS)

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• 作者：Lin-Wei CHEN^a ; ^b ; ^Δ ; Qin WANG^a ; ^Δ ; Kun-Ming QIN^a ; ^b ; Xiao-Li WANG^a ; Bin WANG^a ; Dan-Ni CHEN^a ; Bao-Chang CAI^a ; ^b ; ^{bccai@126.com" class="auth_mail" title="E-mail the corresponding author} ; Ting CAI^c ; ^{tcai@cpu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Qixue Shuangbu Tincture ; UPLC-QTOF/MS ; Chemical profiling ; Traditional Chinese medicine
• 刊名：Chinese Journal of Natural Medicines
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：14
• 期：2
• 页码：141-146
• 全文大小：626 K

文摘

The present study was designed to develop and validate a sensitive and reliable ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) method to separate and identify the chemical constituents of Qixue Shuangbu Tincture (QXSBT), a classic traditional Chinese medicine (TCM) prescription. Under the optimized UPLC and QTOF/MS conditions, 56 components in QXSBT, including chalcones, triterpenoids, protopanaxatriol, flavones and flavanones were identified and tentatively characterized within a running time of 42 min. The components were identified by comparing the retention times, accurate mass, and mass spectrometric fragmentation characteristic ions, and matching empirical molecular formula with that of the published compounds. In conclusion, the established UPLC-QTOF/MS method was reliable for a rapid identification of complicated components in the TCM prescriptions.