A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells
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- 作者：Judd F. Hultquist1 ; 2 ; 3 ; 16 ; Kathrin Schumann4 ; 5 ; 16 ; Jonathan M. Woo4 ; 5 ; 6 ; Lara Manganaro7 ; Michael J. McGregor1 ; 2 ; 3 ; Jennifer Doudna6 ; 8 ; 9 ; 10 ; 11 ; Viviana Simon7 ; 12 ; 13 ; Nevan J. Krogan1 ; 2 ; 3 ; nevan.krogan@ucsf.edu" class="auth_mail" title="E-mail the corresponding author ; Alexander Marson4 ; 5 ; 6 ; 14 ; 15 ; 17 ; alexander.marson@ucsf.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：CRISPR/Cas9 ; HIV integrase ; primary T cells ; genome editing ; CXCR4 ; CCR5 ; LEDGF ; TNPO3 ; host-pathogen interactions ; host dependency factors
- 刊名：Cell Reports
- 出版年：2016
- 出版时间：25 October 2016
- 年：2016
- 卷：17
- 期：5
- 页码：1438-1452
- 全文大小：3145 K
文摘
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Cas9 RNP editing of CXCR4 and CCR5 protects primary human T cells from HIV
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Arrayed delivery of RNPs confirms LEDGF and TNPO3 as HIV dependency factors
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Method allows for efficient, one-step generation of double-knockout primary cells
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Screen of 45 predicted integrase interactors validates new HIV host factors