Three novel aptamers against the coat protein (CP) of grouper nervous necrosis virus (GNNV) were selected.
These aptamers could specifically bind the CP with high (nanomolar) affinities.
All the three aptamers showed no cytotoxic effect in vitro and in vivo.
The selected aptamers could inhibit NNV infection in vitro and in vivo.
The aptamers could bind to NNV virions and directly enter NNV-infected cells.